What Will You Learn?

The participant after attending will be able to understand and analyse the various uses of the High-Performance Liquid Chromatography (HPLC), which include:
● Qualitative analysis – Separation of thermally unstable chemical and biological compounds, e.g., drugs (aspirin and ibuprofen), salts (sodium chloride), proteins (egg white or blood), organic chemicals (polystyrene and polyethylene), herbal medicines, and plant extracts.
● Quantitative analysis – To determine the concentration of a compound in a sample by measuring the height and area of the chromatographic peak.
● Preparation of pure substances for clinical and toxicology studies and in organic synthesis. This is also called preparative chromatography.
● Trace analysis – this is the analysis of compounds present in very low concentrations in a sample.
● This is very useful in pharmaceutical, toxicology, environmental, and biological studies.